Conjugation of Amphiphilic Proteins to Hydrophobic Ligands in Organic Solvent
نویسندگان
چکیده
منابع مشابه
Complexation and conjugation approaches to evaluate siRNA delivery using cationic, hydrophobic and amphiphilic peptides.
In this study, we used solid phase synthesis to prepare three kinds of peptides and then formulated their peptide-siRNA complexes and peptide-siRNA conjugates. Both the complexation and conjugation systems were nontoxic and allowed the delivery of siRNA into the cytoplasm without the need for any transfection agents and with subsequent inhibition of gene expression.
متن کاملExtending the Hydrophobic Mismatch Concept to Amphiphilic
2 Membranolytic Peptides 3 Ariadna Grau-Campistany,‡,† Erik Strandberg,‡ Parvesh Wadhwani,‡ Francesc Rabanal,† 4 and Anne S. Ulrich*,‡,§ 5 ‡Institute of Biological Interfaces (IBG-2), Karlsruhe Institute of Technology (KIT), POB 3640, 76021 Karlsruhe, Germany 6 †Departament de Química Orgaǹica, Facultat de Química, Universitat de Barcelona, Barcelona, Spain 7 Institute of Organic Chemistry, KIT...
متن کاملSolvent-tunable binding of hydrophilic and hydrophobic guests by amphiphilic molecular baskets.
[reaction: see text] Responsive amphiphilic molecular baskets were obtained by attaching four facially amphiphilic cholate groups to a tetraaminocalixarene scaffold. Their binding properties can be switched by solvent changes. In nonpolar solvents, the molecules utilize the hydrophilic faces of the cholates to bind hydrophilic molecules such as glucose derivatives. In polar solvents, the molecu...
متن کاملSynthesis of Apolipoprotein B Lipoparticles to Deliver Hydrophobic/Amphiphilic Materials
To develop a drug delivery system (DDS), it is critical to address challenging tasks such as the delivery of hydrophobic and amphiphilic compounds, cell uptake, and the metabolic fate of the drug delivery carrier. Low-density lipoprotein (LDL) has been acknowledged as the human serum transporter of natively abundant lipoparticles such as cholesterol, triacylglycerides, and lipids. Apolipoprotei...
متن کاملHydrogen exchange in proteins as a measure of solvent exclusion due to ligands. Nuclease and myoglobin.
The kinetic distribution of hydrogen atoms back-exchanging from tritium-labeled proteins has been estimated from the results of interrupted flow and slow, continuousflow gel filtration. Staphylococcal nuclease exchanges almost all of it bound tritium atoms in a few hours at pH 8.1, but in the presence of calcium ions and deoxythymidine 3’,5’-diphosphate about 34 hydrogen atoms remain associated...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bioconjugate Chemistry
سال: 2018
ISSN: 1043-1802,1520-4812
DOI: 10.1021/acs.bioconjchem.8b00354